Nzira yekusarudza sero tsika zvinodyiwa?
1. Sarudza nzira yekurima
Zvinoenderana nemhando dzakasiyana dzekukura, maseru akakamurwa kuita mapoka maviri: adherent masero uye kuturika maseru, uye kune zvakare maseru anogona kukura mune ese adherent uye kuturika, akadai seSF9 masero.Masero akasiyana ane zvakasiyana zvinodiwa kuti sero tsika consumables.Masero anoteera anowanzo shandisa TC-yakarapwa zvinobatika, nepo kumiswa maseru haana zvinodiwa zvakadaro, asi TC-yakarapwa consumables yakakodzerawo kumiswa kwesero kukura.Kuti usarudze zvakakodzera zvinodyiwa, iyo sero tsika nzira inofanirwa kutanga yatemwa zvichienderana nerudzi rwesero.
2. Sarudza rudzi rwezvinhu zvinoshandiswa
Zvakajairwa maseru tsika zvinodyiwa zvinosanganisira sero tsika ndiro, sero tsika ndiro, sero tsika square flasks, cell roller bhodhoro, mafekitori esero,Serological pipettes, etc. Izvi zvinodyiwa zvine maitiro azvo maererano nenzvimbo yetsika, nzira yekushandisa, uye chimiro chese.Bhodhoro retsika tsika yakavharwa, iyo inogona kuderedza kusvibiswa;ndiro yetsika uyepetri dishitsika-yakavhurika tsika, iyo yakanakira kudzora zviedzo uye gradient kuyedza, asi zvakare inogona kukonzera kusvibiswa kwebhakitiriya, izvo zvinoda vashandi vepamusoro.Zvimwe zvekushandisa zvinodawo kushandiswa nemichina yakakosha.Semuenzaniso, sero shaker inoda kushandisa vibrate yeshaker kuita kuti masero abatane nemhepo, uye 40-layer cell fekitori inoda otomatiki michina.Muchidimbu, kana uchisarudza rudzi rwezvinodyiwa, zvinofanirwa kutariswa zvizere pamwe chete nezvido zvekuyedza uye zvido zvemunhu zvekushandisa.
1.Multi-zvakanakasero tsika Mahwendefa: Mafomati etsika dzemasero anoshandisa mahwendefa etsika emhando dzakawanda ari kuwedzera kukurumbira nekuti anofambisa kudzidza kwemhando dzakasiyana-siyana, kuderedza nguva yekuedza, uye kuchengetedza zvinodhura.Kuwedzera kune akajairwa akakwira-kuburikidza madiki-mahwendefa, akakosha madiki-mahwendefa akagadzirwa kuti afambise 3D uye organotypic cell tsika.
1) Nhamba yemakomba
Zvinoenderana neinodiwa flux level, uye kana pasina rubatsiro rwemuchina.6, 12, 24 uye mamwe mashoma tsime tsika mahwendefa anogona kuwedzerwa nemaoko.Ku96-zvakanakasero tsika ndiro, zviri nani kuva nerubatsiro rwepipette yemagetsi kana muchina.
2) Chimiro chegomba
Pasi pegomba rinogona kusarudzwa kuti rive rakatsetseka (F-pasi), denderedzwa (U-pasi), kana kuti tapered, zvichienderana nerudzi rwesero uye pasi pekushandisa.
3) Ruvara rwendiro
Iyo ruvara rweperforated plate inobatanawo zvakanyanya nekushandisa.Kana maseru akacherechedzwa nechikamu chekusiyanisa maikorosikopu kana neziso rakashama, inoonekera yakawanda-tsime cell culture plate inogona kusarudzwa.Nekudaro, kune maapplication ari kunze kweinooneka mwenje spectrum (senge luminescence kana fluorescence), ine mavara akawanda-zvakanaka.sero tsika ndiro(zvakadai sechena kana dema) zvinodiwa.
4) Kurapa kwepamusoro
Ndeupi sero mushonga wepamusoro wekusarudza unoenderana nekuti uri kurima kumiswa here kana kutevedzera maseru.
2.Sero tsika flasks: Nzvimbo yetsika inotangira kubva ku25-225 cm², uye inowanzo gadziriswa pamusoro, inokodzera kunamatira kwesero uye kukura.225cm² uye 175cm²sero tsika flasksanonyanya kushandiswa kune hombe-tsika tsika (senge monoclonal cell tsika, nezvimwewo), 75cm² inonyanya kushandiswa kuyedza masero (general ndima, kuchengetedza masero, maseru ekuedzwa, nezvimwewo), 25cm² inowanzoshandiswa kuInogona kushandiswa kumutsidzira masero kana tsika kana paine mashoma maseru, uye pakugadzira macell cell, akawanda mabhodhoro anogona kushandiswa kudzivirira kusvibiswa kwemuchinjikwa.
3.Erlenmeyer flask: Zvichienzaniswa nezvinodyiwa zvakaita semafekitori emasero uye sero roller bhodhoro, ine diki sero tsika nharaunda uye iri ehupfumi sero tsika chishandiso.Iyo bhodhoro muviri weflask wakagadzirwa ne polycarbonate (PC) kana PETG zvinhu.Iyo yakasarudzika yematatu maumbirwo ekugadzira inoita kuti zvive nyore kuti pipette kana sero scraper isvike pakona yebhodhoro, zvichiita kuti sero tsika yekushanda zvive nyore.Theerlenmeyer flaskchivharo chinogadzirwa nepamusoro-simba HDPE zvinhu, iyo yakakamurwa kuita chisimbiso chivharo uye chivharo chinofema.Chisimbiso chivharo chinoshandiswa kune yakavharwa tsika yegesi uye mvura.Iyo inofema cap inoshongedzerwa ne hydrophobic sefa membrane pamusoro pevharo rebhodhoro.Inodzivirira kupinda uye kubuda kwehutachiona, inodzivirira kusvibiswa, uye inovimbisa kuchinjanisa gasi, kuitira kuti masero kana mabhakitiriya akure zvakanaka.
Iwo akajairika saizi econical shakeerlenmeyer flasksari 125ml, 250ml, 500ml, 1000ml uye3L, 5L yakakwirira kunyatsoshanda erlenmeyer flasks, Kuti uone kukwanisa kwepakati uye kubata mamiriro ekukura kwemasero, chiyero chichadhindwa pamutumbi webhodhoro.Tsika dzemasero dzinoda kuitwa munzvimbo isina utachiona.Naizvozvo, iyo Erlenmeyer flask ichaitwa yakakosha sterilization kurapwa isati yashandiswa kuti iwane mhedzisiro yekusava neDNase, hapana RNase, uye hapana inotorwa nemhuka, inopa mamiriro akanaka ekukura kwesero.zvakapoteredza.
4.Multi-layerCell Factory: Fekitari yemasero Inokodzera kugadzirwa kwemaindasitiri emabhechi, senge majekiseni, monoclonal antibodies kana indasitiri yemishonga, asiwo yekushanda kwerabhoritari uye yakakura-chiyero masero tsika.Zviri nyore uye zvinoshanda, zvinobudirira kudzivirira kusvibiswa.Cell Factory ine Chivharo Yakavharwa: Kavha yacho haina maburi ekufema, uye inonyanya kushandiswa pasi pemamiriro asina kabhoni dhaidhiyoni akadai sema incubators uye greenhouses.Cell Factory ine chivharo chakavharwa inogona kudzivirira kupinda kwekunze kwebhakitiriya uye kubatsira kugadzira nzvimbo yakanaka yekukura yekukura kwesero.Chifukidziro chinofema: Kune maburi ekufema pamusoro pekavha, ayo anonyanya kushandiswa munzvimbo yecarbon dioxide.Makomba ekufemesa anobvumira kabhoni dhaikisaidhi munharaunda kuti ipinde mufekitari yesero, ichigadzira mamiriro ekukura akakodzera ekukura kwesero.Pane 1 layer,2 layer,5 layers,10 layers,40 layersmafekitari emaseroiripo.
5.Sero tsikaroller bhodhoro: 2L & 5L Roller mabhodhoro Inokodzera zvakasiyana-siyana zvinonamatira sero tsika uye Suspension cell tsika, kusanganisira Vero masero, HEK 293 masero, CAR-T masero, MRC5, CEF masero, porcine alveolar macrophages, myeloma masero, DF-1 masero, ST masero, PK15 masero, Marc145 masero mamwe adherent masero.Izvo zvakakodzerawo kune static tsika yekusimudzira masero akadai seCHO masero, masero ezvipembenene, BHK21 masero uye MDCK masero.
3.Sarudza zvakatemwa zvekushandisa.
Huru-mwero sero tsika kuedza zvinoda consumables ane hombe tsika nzvimbo yerutsigiro, nepo diki-mwero kuedza kusarudza consumables ane diki nzvimbo.Mafekitari emasero anonyanya kushandiswa kune yakakura-yakakura sero tsika, sekugadzirwa kwejekiseni, monoclonal antibodies, indasitiri yemishonga, nezvimwewo;tsika ndiro, ndiro, uye flasks akakodzera diki-mwero sero tsika mumarabhoritari;kunze kwekumisikidza sero tsika, iyo flask inogona zvakare Kwepakati kugadzirira, kusanganisa uye kuchengetedza.Zvinoenderana nesero tsika chikero, sarudza zvakatemwa zvezvinhu zvinodyiwa.
Zvakakodzera sero tsika consumables ndiwo musimboti kuona zvakanaka sero kukura, uye zvakare kiyi kukurumidza kuyedza maitiro uye kuona tsika mhedzisiro.Mukusarudza, zvinhu zvakaita senzira yetsika nemagariro, chiyero chetsika, uye mamiriro emurabhoritari anofanirwa kutariswa zvizere.isu tinofanirwa kushandisa zvimwe zvinoshandiswa pakuita sero tsika, semuenzaniso,flake mutakuri weCellDisk&spherical mutakuri weCellDisk,pipette mazano,firimu rekusimbisa,pipettes, nezvimwewo, Luoron inogonawo kupa.
LuoRon Biotech Co, Ltd inotarisa pakutsvagisa, kusimudzira, kutengesa uye sevhisi yezvinhu zvipenyu zvinodyiwa.Fekitori yekugadzira ine nzvimbo yekudyara ye10,000 square metres.Iyo ine giredhi 100,000 yakachena yekugadzira workshop, giredhi 10,000 level yemusangano musangano uye yakakwirira-chaiyo mold tsvakiridzo uye musangano wekugadzira.
Muchidimbu, kana uchisarudza rudzi rwezvinodyiwa, zvinofanirwa kutariswa zvizere pamwe chete nezvido zvekuyedza uye zvido zvemunhu zvekushandisa.Ehe, zvakakosha zvakaenzana kusarudza chikuva chakaita seLuoRon ine zvigadzirwa zvepamusoro-soro uye zvakasiyana-siyana, kuwanikwa kwakagadzikana, kuvimbiswa kwemhando uye sevhisi.LuoRon inogona kupa huwandu hwakazara hweimwe-stop yekutenga masevhisi ezvekutsvagisa zvesainzi zvemurabhoritari mumunda wesainzi yehupenyu hwepasirese, indasitiri yemishonga, kuchengetedza kwezvakatipoteredza, kuchengetedza chikafu, masangano ehurumende, uye mishonga yekiriniki.
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