Theerlenmeyer shake flaskchinhu chakakosha chetsika chigadziko chekumisikidza sero tsika, uye chinogona zvakare kushandiswa kugadzirira, kusanganisa uye kuchengetedza akasiyana midhiya.Iyi tsika inoshandiswa inoshandiswa pakugadzira masero eJurkat.

Iyo Jurkat cell line inotorwa kubva muropa reparipheral remukomana ane makore gumi nemana uye iri sero rakamiswa.Jurkat-yakatorwa sero mitsara isina mamwe majini yatovepo mumabhengi etsika tsika.Mitsetse yeT lymphocyte isingafe yemunhu inonyanya kushandiswa kudzidza acute T cell leukemia, T cell signing, uye akasiyana chemokine receptors anotarirwa kupinda nehutachiona, kunyanya kutaura kweHIV.Iine huwandu hwakakura kwazvo hwekushandisa mukutsvagisa zvipenyu, sekushandiswa kweJurkat masero kudzidza iyo M1-RNA yeribonuclease P, uye kudzidza kweM1-RNA yeAnti-MHC class II transcriptal activator (CIITA) kuti inhibit. kutaura kweMHC kirasi II mamorekuru pane cell cell.

Paunenge uchigadzira masero eJurkat muerlenmeyer shake flasks, RPMI1640 yepakati, 10% FBS inodiwa;tembiricha inodzorwa pa 37 ° C, 5% kabhoni dhaibhorosi, PH kukosha 7.2-7.4, aseptic inogara tembiricha tsika.Pukuta uye kuuraya utachiona ne75% doro usati waendesa kuchitokisi ultra-clean bhenji, tora sero cryovial kubva mumvura ine nitrogen tangi, pakarepo uiise mumvura yekugezera ye37 ° C uye zunza sero cryotube nekukurumidza kuti inyunguduke nekukurumidza.Zvadaro, mushure me centrifugation, pipetting uye kusanganisa, nezvimwewo, yakaiswa mu cell incubator yekurima.

Masero anonyanya kufarira zvakatipoteredza.Paunenge uchigadzira masero eJurkat muerlenmeyer cell shake flasks, hutsanana hwemunhu hunofanirwa kuitwa nemazvo, mareagents asina kuchena anofanirwa kushandiswa, uye misimboti yekushanda kweaseptic inofanirwa kuteverwa kudzivirira kuunza mabhakitiriya uye kukanganisa kukura kwesero.