PETG tsika yepakati bhodhoroibhodhoro repurasitiki rinoshandiswa zvakanyanya.Muviri wayo webhodhoro wakajeka zvakanyanya, unotora dhizaini dhizaini, huremu huremu, uye haisi nyore kutyora.Igaba rakanaka rekuchengetedza.Zvishandiso zvedu zvakajairika zvinonyanya zvinotevera zvitatu:

1. Serum: Serum inopa masero ane zvakakosha zvinovaka muviri, kukura zvinhu, kusunga mapuroteni, nezvimwewo, kudzivirira kukuvara kwemagetsi kumasero, uye kudzivirira masero mutsika.Serum yekuchengetera kwenguva refu inofanirwa kuchengetwa munzvimbo yakadzikama tembiricha ye -20°C kusvika -70°C.Kana yakachengetwa mufiriji ye4°C, kazhinji isingapfuuri mwedzi mumwe.

2.Culture medium: Hunhu huzhinji hune makabhohaidhiretsi, nitrogenous zvinhu, inorganic salts, mavhitaminzi nemvura, etc. Haisi chete chinhu chakakosha chekupa sero kudya uye kukurudzira kuwanda kwesero, asiwo nharaunda yekugara yekukura kwesero uye kuberekana. .Nzvimbo yekuchengetedza yepakati ndeye 2 ° C-8 ° C, yakachengetedzwa kubva pachiedza.

3. Various reagents: Pamusoro pekuchengetwa kweserum uye tsika yepakati, PETG mabhodhoro epakati anogonawo kushandiswa semidziyo yekuchengetedza yezvakasiyana-siyana biological reagents, zvakadai sebuffers, dissociation reagents, antibiotics, cell cryopreservation solutions, staining solutions, Growth additives, etc. Zvimwe zveizvi reagents zvinoda kuchengetwa pa -20 ° C, asi zvimwe zvakachengetwa mukamuri yekupisa.Hazvina mhosva kuti inzvimbo ipi, bhodhoro repakati rinogona kusangana nezvinodiwa zvekuchengetedza.

Iyo PETG yepakati bhodhoro inonyanya kushandiswa kubata mhinduro nhatu dziri pamusoro.Kuti zvive nyore kutarisa kutaridzika kwehuwandu hwemhinduro, pane chiyero pamutumbi webhodhoro.Mhinduro dziri pamusoro dzinonyanya kushandiswa musero tsika, uye kutarisisa kunofanirwa kubhadharwa kune aseptic mashandiro kana uchivawedzera.