Mukumiswa kwesero tsika,cell shake flaskrudzi rwesero tsika inodyiwa.Kukura kwemaseru akaturikwa kwaisaenderana nepamusoro pezvinhu zvinotsigira uye akakura mukumiswa mamiriro mutsika yetsika.Tinoziva sei huwandu hwemvura inofanira kuwedzerwa mutsika chaidzo?

Zvakajairwa kutaurwa kwesero shaker zvinosanganisira 125ml, 250ml, 500ml uye 1000ml kusangana nezvinodiwa zvehukuru hwakasiyana hwesero tsika.Semuenzaniso, 125ml uye 250ml mabhodhoro ane hudiki hudiki anonyanya kushandiswa padiki-diki kuyedza, nepo 500ml uye 1000ml kutsanangurwa kunoshandiswa kune yepakati-mwero sero tsika kuyedza.Paunenge uchishandisa rudzi urwu rwekushandisa, iyo vibrate ye shaker inofanirwa kushandiswa kudzikisa iyo cell agglomeration rate uye kuchengetedza yakanaka kukura mamiriro emaseru.Tsika dzemasero dzinoda kuitirwa munzvimbo isina utachiona.Naizvozvo, the triangle culture flask ichave yakanyatso itwa sterilized isati yashandiswa kuti iwane mhedzisiro yekusava neDNase, hapana RNA enzyme, uye hapana zvikamu zvakatorwa nemhuka, zvichipa nharaunda yakanaka yekukura kwesero.

Zvinoenderana nekutsanangurwa kwakasiyana kwebhodhoro, iyo yakakurudzirwa yekuzadza vhoriyamu yezvina zvakatemwa zvebhodhoro kubva pasi kusvika kumusoro ndeye 30ml, 60ml, 125ml, 500ml.Kazhinji, vhoriyamu yemhinduro mutsika yemasero inodzorwa ingangoita 20% -30% yehuwandu hwehuwandu hwebhodhoro rinozunguza, uye pane chiyero chakajeka mutsara pane bhodhoro muviri kufambisa kutarisisa kwekuona kugona kwemhinduro. .

Iyo iri pamusoro ndiyo yakakurudzirwa huwandu hwemvura yakawedzerwa kune akasiyana zvirevo zve cell shaker, iyo isina kugadzikwa.Kugona kunofanirwa kutariswa zvakazara zvichienderana nehunhu hwekukura kwesero uye inoculation density, kuitira kudzivirira kufurira kwekukura kwesero nekuda kwekuwanda kwemvura yakawedzerwa.