Kamwe maseru isu tsika mukati

sero fekitariakasvibiswa, mazhinji acho akaoma kubata.Kana masero akasvibiswa akakosha uye akaoma kuwana zvakare, nzira dzinotevera dzinogona kushandiswa kuabvisa.

1. Shandisa mishonga inorwisa mabhakitiriya

Mishonga inorwisa mabhakitiriya inoshanda zvakanyanya pakuuraya mabhakitiriya mukatimafekitari emasero.Mushonga wekusanganiswa unoshanda kupfuura mushonga wega.Mushonga wekudzivirira unoshanda kupfuura mushonga mushure mekusvibiswa.Mushonga wekudzivirira unowanzo shandisa mishonga inorwisa mabhakitiriya kaviri (penicillin 100u/mL pamwe nestreptomycin 100μg/mL).Mushure mekusvibiswa, nzira yekuchenesa inoda kuva 5 kusvika ku10 nguva huru pane yakajairika huwandu.Mushonga unofanirwa kushandiswa kwemaawa makumi maviri nemana kusvika makumi mana nemasere mushure mekuwedzera, uye wozotsiviwa nemaitiro akajairika.Culture fluid.Iyi nzira inogona kushanda mumatanho ekutanga ekusvibiswa.Mukuwedzera kune penicillin uye streptomycin, mishonga inorwisa mabhakitiriya inoshandiswa inogonawo kusanganisira gentamicin, kanamycin, polymyxin, tetracycline, nystatin, nezvimwewo. Inowanzoshandiswa ndeye 400 kusvika 800 μg / mL kanamycin kana 200 μg / mL tetracycline.Iyo svikiro inochinjwa mazuva maviri kusvika matatu oga oga uye inopfuudzwa kwechizvarwa chimwe kusvika ku2 kuti irapwe.Mumakore achangopfuura, zvakataurwa kuti 4-fluoro, 2-hydroxyquinoline (Ciprofloxacin, Cip), Pleu-romutilin derivative (Pleu-romutilin derivative, BM-Cyclin2: BM-1 uye tetracycline derivative (BM-2)) Mishonga inorwisa mabhakitiriya inoshanda pakuuraya mycoplasma kana ichishandiswa yoga kana pamwe chete.Aya maantibayoti matatu anogadzirwa ese ari 250X concentrated solutions muPBS uye anochengetwa pa -20°C kuti azoshandiswa gare gare.Iko kushandiswa kwekugadzirisa Cip ndeye 10 μg/mL, BM-1 i10 μg/mL, uye BM-2 i5μg/mL.Paunenge uchishandisa, tanga washuvira iyo yakasvibiswa tsika yepakati, wedzera RPMI1640 tsika yepakati ine BM-1, wozoshuvira tsika yepakati mushure memazuva matatu, wedzera RPMI1640 tsika yepakati ine BM-2, uye tsika kwemazuva mana, zvichingodaro kwemazuva matatu akateedzana. .kutenderera, kusvika zvaratidzwa ne33258 fluorescent staining microscopy kuti mycoplasma yakabviswa, ipapo yakajairika tsika svikiro inowedzerwa kune tsika uye ndima 3-4 nguva.

2. Kupisa kurapwa

Kuisa tsika yenyama yakasvibiswa pa41 ° C kwemaawa gumi nemasere inogona kuuraya mycoplasma, asi ine mhedzisiro yakaipa pamaseru.Naizvozvo, bvunzo yekutanga inofanira kuitwa isati yarapwa kuti iongorore nguva yekupisa iyo inogona kuuraya mycoplasma kusvika pamwero mukuru uye ine zvishoma zvinokanganisa maseru.Iyi nzira dzimwe nguva haina kuvimbika.Kana ukarapwa nemishonga kutanga uye wozopisa pa41 ° C, mugumisiro uchava nani.

3. Shandisa mycoplasma-specific serum

Kusvibiswa kweMycoplasma kunogona kubviswa ne5% rabbit mycoplasma immune serum (hemagglutination titer 1:320 kana pamusoro).Nekuda kwekuti masoja ekudzivirira chirwere anogona kutadzisa kukura kwe mycoplasma, inoshanduka kuita isina kunaka mazuva gumi nerimwe mushure mekurapwa kweantiserum uye inoramba isina kunaka mushure memwedzi mishanu.ari negative.Zvisinei, nzira iyi inonyanya kunetsa uye haisi nyore uye ine mari sekushandisa mishonga inorwisa mabhakitiriya.

4. Dzimwe nzira

Pamusoro penzira dzataurwa pamusoro apa dzekubvisa kusvibiswa, kune zvakare inoculation uye sterilization nzira mumhuka, macrophage phagocytosis nzira, nzira dzekuwedzera bromouracil kune yakasvibiswa.tsika mabhodhorowobva waavheneka nechiedza, uye nzira dzekusefa, nezvimwewo, asi zvese zvinonetsa uye hazvishande.Nokudaro, kana kusvibiswa kwemycoplasma kuchiitika, kunze kwekuti inonyanya kukosha, inowanzoraswa uye inogadzirwa zvakare.

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