Kana isu tikashandisa mamwe maseru tsika zvinodyiwa, isu tinogara tichisangana nedambudziko resero ndima.Nhasi, tichagovana newe muchidimbu mashandisirwo emhando yepamusoro shake flasks ye cell passage.Patinoshandisa high-efficiency shake flasks(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/) ye cell passage, pane nzira mbiri dzaungasarudze kubva, sekuunganidza maseru necentrifugation wozopfuura, kana kunanga. ndima.

Centrifugal ndima nzira:

(1) Shandura maseru ari muhigh-efficiency shake flask pamwe chete netsika yepakati kune centrifuge chubhu ye centrifugation.

(2) Rasa mashura, wedzera tsika itsva yepakati kune centrifuge chubhu uyepipettekugadzira sero kuturika.

(3) Verenga uye jekiseni mune itsva tsika flasks maererano.

Kana nzira yakananga yakagamuchirwa, rega masero akarembera agare zvishoma nezvishoma pazasi pe-high-efficiency shake flask, kuyamwa kubva 1/2 ~ 2/3 ye-supernatant, uye ipapo pipette kuti iite kumisa sero isati yapfuura.

Chatinofanira kutarisisa panguva yekuvhiya ndechekuti trypsin inofanira kunge isati yadziya, uye tembiricha inenge 37°C.Iyo centrifugation yekumhanya inofanirwa kuve yakakodzera.Kana kukurumidza kuderera, masero haagoni kuparadzaniswa zvakanaka.Kana iyo centrifugation yekumhanyisa yakakwira uye nguva yakarebesa, maseru anosvinwa, zvichikonzera kukanganisa kana kufa.Masero anofanirwa kucherechedzwa nguva dzose, uye kana kusvibiswa kunowanikwa, kunofanirwa kugadziriswa nenguva.