Liquid strain idhizaini rakasimudzwa rine liquid culture medium.Iyo ine mabhenefiti epfupi dhizaini yekugadzira kutenderera, inowirirana zera rebhakitiriya, inoculation iri nyore, yakakodzera kugadzirwa kwefekitori, zvichingodaro.Yakagamuchirwa neruzhinji rwevarimi.Cell shaker (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/） imhando yezvinhu zvinodyiwa zvinodikanwa mutsika yemhando dzemvura.Aya mapoinzi anotevera anofanirwa kutariswa mune tsika:

1.Kugadzirira kwetsika yepakati maererano nekugadzirwa kwezvinhu zvakasiyana-siyana, mumudziyo, kunyunguduka nemvura, yakakamurwa kuva mabhodhoro matatu, kazhinji 250 ~ 300mL cell shake bhodhoro 50mL tsika yepakati,500mL katatu bhodhoro  (https://www.luoron.com/plastic-erlenmeyer-flask-with-vent-cap-2-product/）100mL tsika yepakati, plug donje plug, nepepanhau rakaputirwa;Inogonawo kugadzirwa ne8 akaturikidzana egauze 8cm × 8cm bhodhoro chivharo chisimbiso, kunze nebrown bepa chisimbiso.
2. Autoclave sterilization nzira inowanzo shandiswa pakuita sterilization, inoda 121 ℃ uye 30min.Bvisa uye utonhodze kusvika pa30 ℃.Iise muimba isina utachiona kana inoculation box kuti ishandiswe.
3. Zvinoenderana neaseptic oparesheni zvinodiwa, bhodhoro rega rega rinogona kubatanidzwa ne 2 ~ 3cm2 slant strain, uye yega yega slant strain inogona kubatanidzwa nemabhodhoro 4 ~ 5.Zviri nani kuunza imwe svikiro neakawedzera madhiri, kuitira kuti vakwanise kuyangarara pamusoro pepakati.Mushure meinoculation, vhara muromo webhodhoro ne gauze pakutanga yakavharwa, uye uisungirire zvakasimba netambo.
4. Iyo inoculated strain bhodhoro inogona kuiswa mumubhedha unozununguka nekuda kwetsika, kana inogona kuiswa mune inogara tembiricha ye24 ~ 26 ℃ ye48h, uye ipapo oscillated tsika inogona kuitwa mushure mekunge aerogenic mycelium yawedzerwa mutsika yepakati. .Iyo oscillating frequency yekudzoreredza shaker ndeye 80 ~ 120r / min, uye iyo ye rotary shaker ndeye 150 ~ 220r/min.3 ~ 4d inogona kugadzirwa mune shaker.Pakupera kwetsika, nekuda kwemhando dzakasiyana, tsika yemagariro yakaonekwa mavara akasiyana, akadai sehowa oyster, enoki mushroom tsika yepakati yaive yero yero;Mushroom, ericius erinaceus culture medium yaive tsvuku tsvuku, uye howa hwema;Iyo fungus culture medium ibrown, gobvu uye kutapira.Iyo tsika yepakati inogona kushandiswa kugadzira kana kuwedzera tsika yekuwedzera chete kana huremu hwakaoma hwemycelium hwasvika 10g/L uye dhayamita ye mycelium pellets iri 1 ~ 2mm.
 
5. Secondary liquid strain kugadzirwa kwechipiri mvura strain tsika yepakati kugadzirwa kweiyo nhanho yemhando yemvura, chigadziko chetsika chinofanira kunge chakakura, huwandu hahupfuuri 3500mL.Mushure mekuita sterilization uye kutonhora, iyo yakaviriswa yemhando yemvura inoiswa mukati5L cell shaker(https://www.luoron.com/3l5l-high-efficiency-erlenmeyer-flask-product/）pachiyero che5% -10% uye yakasimwa pane shaker nekumhanya kunononoka.Mushure meimwe nguva yehutsika hutsika, mvura inoyerera ine yunifomu yekugovera microspheres uye yakajeka yekuvirisa mvura inogona kuwanikwa.
Muchidimbu, kana uchishandisa sero shaker kune tsika yemvura strains, shanda zvinoenderana nepamusoro nzira, uye teerera kudzora tembiricha, kuzunungusa frequency uye zvimwe zvinhu mukuita kuti uve nechokwadi chetsika.
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