Shake flask tsikairi padanho rekunetsekana kuongorora uye tsika (mutyairi bvunzo), mamiriro etsika anofanirwa kunge ari padyo sezvinobvira kune inovirisa kugadzirwa kwetsika mamiriro, basa rakakura, nguva refu, kushanda kwakaoma.Zvinhu zvinokanganisa kushanda kwepamusoro kwekuzunungusa flask tsika zvinonyanya kupisa tembiricha, kuzunungusa amplitude ye shaker, kuwanda kwekuzunguza flask, iyo pH yetsika yepakati, viscosity yepakati, etc. Tsika tembiricha: the mycelium kukura tembiricha. emhando dzakasiyana dzefungi dzinodyiwa zvakare dzakasiyana, yakawanda yeakakodzera tembiricha yekukura iri pakati pe22 ℃ uye 30 ℃, kana tembiricha yetsika yakadzikira, mycelium kukura kunononoka;Apo tembiricha yakanga yakanyanyisa, mycelium pellets yakanga yakasununguka uye shoma, uye simba nemhando ye mycelium pellets yakaderera.

Kuzunungusa frequency uye kugona kwakanyanya kuzunza bhodhoro kurodha: fungi inodyiwa ndeye aerobic fungi, yemvura tsika, kunyanya nekutorwa kweokisijeni yakanyungudika mukati metsika.Iyo okisijeni yakanyungudika mutsika yepakati inonyanya kukanganiswa neiyo viscosity yepakati, huwandu hwemvura mumudziyo, kuwanda kweiyo oscillation uye zvimwe zvinhu.Iko kuzununguka kwakawanda, bhodhoro rekudengenyeka idiki, kusungirirwa kwepakati kunosvika, iyo yakanyungudutswa okisijeni yepakati yakakwira, uye imwe nzira yakapoteredza yakaderera.Kazhinji rotary shaker kumhanya ndeye 180-220 RPM / min, kudzokorora ndeye 80-120 RPM / min, amplitude 6-7cm.

Ph yetsika yepakati: PH yetsika yepakati inobata zvakananga kunwisa, enzyme chiitiko uye mycelial pellet kukura.Yakananga pH inofanirwa kugadziridzwa isati yaitwa sterilization, fungus zhinji dzinodyiwa mupH 2.0-6.0.Kuti udzivise kushanduka kwakanyanya kwePH mutsika yemagariro, calcium carbonate, phosphate uye zvimwe zvinhu zvebuffer zvinowanzowedzerwa kune tsika yepakati.

Medium viscosity: Medium viscosity inokanganisa zvakananga huwandu hweokisijeni yakanyungudika mairi, uye inokanganisawo kuumbwa kwe mycelial pellets.Zvigumisiro zvakaratidza kuti apo viscosity yetsika yepakati yakawedzera, dhayamita ye mycelium pellets yakaderera, nhamba yakawedzera, uye goho rinowedzera.Naizvozvo, maererano nezvinodiwa zvemvura inoyerera padhayamita ye mycelium pellets, tsika yepakati ine imwe viscosity inofanirwa kugadzirwa.Sero tsika ibasa rakaoma, kunyanya kana richida kugadzirwa nerubatsiro rweshaker, senge yakakwira-inoshanda shaker, inofanirwa kutariswa zvakanyanya, kuitira kuti ive nechokwadi chekufambira mberi kwakanaka kwesero tsika.