Sero fekitari chinhu chakajairika chinodyiwa muhukuru hwesero tsika, iyo inonyanya kushandiswa kune adherent cell tsika.Kukura kwesero kunoda marudzi ese ezvinovaka muviri, saka zvii?
1. Chimiro chetsika
Sero tsika yepakati inopa masero mufekitari yesero ine chikafu chinodiwa pakukura, kusanganisira makhahydrates, amino acids, inorganic salts, mavhitaminzi, etc. Kune zvakasiyana-siyana zvekugadzira media zvinowanikwa kune zvinodiwa zvekudya zvemasero akasiyana, akadai seEBSS. , Gondo, MEM, RPMll640, DEM, nezvimwewo.

2. Zvimwe zvinowedzerwa
Mukuwedzera kune zvakakosha zvinovaka muviri zvinopihwa neakasiyana ekugadzira media, zvimwe zvikamu, senge serum uye zvinhu, zvinoda kuwedzerwa zvinoenderana nemasero akasiyana uye akasiyana tsika zvinangwa.
Serum inopa zvinhu zvakakosha senge extracellular matrix, kukura zvinhu uye transferrin, uye fetal bovine serum inowanzoshandiswa.Chikamu cheserum chichawedzerwa chinoenderana nesero uye chinangwa chechidzidzo.10% ~ 20% serum inogona kuchengetedza kukurumidza kukura uye kupararira kwemasero, anozivikanwa sekukura kwepakati;Kuti uchengetedze kukura kunonoka kana kusafa kwemasero, 2% ~ 5% serum inogona kuwedzerwa, inonzi tsika yekuchengetedza.
Glutamine yakakosha nitrogen sosi yekukura kwesero uye inoita basa rakakosha mukuita kwekukura kwesero uye metabolism.Nekudaro, nekuti glutamine haina kugadzikana uye iri nyore kudzikisira mumhinduro, inogona kuora nezve 50% mushure memazuva manomwe pa4 ℃, saka glutamine inoda kuwedzerwa isati yashandiswa.
Kazhinji, midhiya yakasiyana-siyana uye serum inoshandiswa mumasero tsika, asi kuitira kudzivirira kusvibiswa kwesero panguva yetsika, imwe nhamba yemishonga inorwisa mabhakitiriya, yakadai se penicillin, streptomycin, gentamicin, nezvimwewo, inowedzerwawo kune vezvenhau.