Kune akawanda maseru zvimedu mushure me mechanical lysis ye cell suspension.Nzira yekubvisa sei zvimedu izvi?Ngatitarisei nzira dzakasiyana-siyana:

1. Shandisa dilution nzira.Kana maseru akacheneswa, anogonawo kuwanda, anowedzera kuwanda, uye marara esero achaita zvishoma nezvishoma.
2. Kunewo kugadzirisa kwepanyama.Masero anogara nekukurumidza kupfuura zvimedu zvakawanda: : Fambisa sero kuturika mukaticentrifuge chubhu, uye kana akawanda emasero anonyura, mhinduro yepamusoro inogona kusvetwa, uye yobva yawedzerwa kune tsika yekugadzirisa kumisa masero.Iyi nzira inogona kushandiswa kakawanda, nepo pese paunokwanisa kuona kana ichizadzisa zvaunoda pasi pemaikorosikopu.
3. Low-speed centrifugeinogona kubvisa marara, kazhinji 700g, 5mins
4. Kana centrifuging, pasi pemamiriro ekuti nhamba yemasero yakakwana, kuderedza nguva ye centrifugation, yakadai se5min, 1000rpm, panzvimbo ye3min, 1000rpm, uye bvisa iyo yepamusoro-soro, nokuti necrosis nemarara zvinowanzova mu supernatant!Kuvhiya uku kunongoda kuitwa kamwe chete panguva yekugeza isati yaiswa!

Kunyangwe paine nzira dzakawanda dzekubvisa sero tsvina, iwe uchaona kuti centrifugation ndiyo imwe yenzira dzinoshanda!

Uye nekuti matishu echirimwa nemaseru ane maseru madziro anoumbwa neselulosi, hemicellulose uye pectin, zvinowanzodikanwa kukuya jecha requartz kana girazi poda nemhinduro yakakodzera yekubvisa kana kubata ne cellulase kuti uwane chinangwa.Bacterial cell fragmentation yakanyanya kuoma, nekuti iyo yese bacterial cell wall skeleton inotova covalent bond ye peptidoglycan cystic macromolecules, yakaoma kwazvo.Nzira dzakajairika dzekupwanya mabhakitiriya maseru madziro anosanganisira ultrasonic kupwanya, jecha kugaya, high pressure extrusion kana lysozyme kurapwa.Mushure mekunge matishu nemaseru zvaputswa, buffer yakakodzera inosarudzwa kuti ibudise puroteni inodiwa.Insoluble zvinhu zvakadai semasero zvidimbu zvinobviswa necentrifugekana kusefa.

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