Cell vacuolation inoreva kutaridzika kwevacuoles (vesicles) ehukuru hwakasiyana mucytoplasm uye nucleus yemasero akaparara, uye masero ari cellular kana reticular.Pane zvikonzero zvakawanda zvemamiriro ezvinhu aya.Tinogona kuderedza vacuolation yemasero mucell culture flaskzvishoma sezvinobvira kuburikidza nekushanda kwemazuva ose.

1. Simbisa iyo sero mamiriro: sarudza iyo sero mamiriro usati wagadzira maseru, uye edza kusarudza maseru ane yakakwira chizvarwa nhamba yekurima, kuitira kudzivirira vacuoles nekuda kwekuchembera kwemasero panguva yekurima.

2. Sarudza kukosha kwepH yehutano hwemagariro: simbisa kukodzera kwepH yetsika yepakati uye pH inodiwa nemaseru kuti irege kukanganisa kukura kwesero nekuda kwepH isina kukodzera.

3. Chengetedza trypsin digestion time: kana subculture, sarudza kusanganiswa kwakakodzera kwetrypsin uye sarudza nguva yakakodzera yekudya kwekudya, uye udzivise mabhubhu emhepo akawandisa panguva yekushanda.

4. Cherekedza chimiro chesero chero nguva: Paunenge uchigadzira maseru, tarisa chimiro chesero mucell culture flaskchero nguva kuve nechokwadi chekuti masero anoda chikafu chakakwana uye kudzivirira sero vacuolization nekuda kwekushaikwa kwekudya.

5. Edza kushandisa fetal bovine serum nemhando yakanaka uye yenguva dzose migero, nokuti serum yakadaro yakapfuma mune zvinovaka muviri uye ine zvishoma exogenous stimulating zvinhu, izvo zvinogona kubudirira kudzivisa matambudziko akadaro.

Izvo zviri pamusoro apa zvinogona kuderedza vacuolation yemasero mucell culture flask.Pamusoro pezvo, zvinodikanwa zvesterility zvinofanirwa kunyatsoitwa panguva yekuvhiya kuderedza mukana wekusvibiswa kwakasiyana.Kana maseru akaonekwa aine hutachiona, anofanirwa kuraswa nenguva kuti asakanganisa ongororo dzinotevera.

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