Sero ndima tsika inoreva nzira yekukamura tsika kuita zvidimbu zvidiki uyezve kuiisa mune imwe tsika mudziyo (bhodhoro) kuti ienderere mberi tsika.High kunyatsoshanda cell shakerndeye yakajairika inodyiwa yekumiswa sero tsika, saka sei kushandisa yakakwirira kunyatsoshanda sero shaker ye cell passage?

Nehunhu hwavo, maseru ekumisikidza haabatike, saka hapana ma enzymes anodiwa kuti aabvise kubva pamusoro peiyo yepamusoro-inoshanda shaker.Murabhoritari yakajairika, ndima yakananga uye centrifugal ndima inowanzoshandiswa kuita nzira yemasero akamiswa.Kana masero akaonekwa kuti akakura ne80 kusvika ku90 muzana (sero suspension inoshanduka yero), masero anenge agadzirira kufamba.

Kana masero ari kukura zvakanaka pasi pemaikorosikopu, nzira yakananga inogona kushandiswa.The medium muhigh-efficiency yekuzunungusa flaskyakakamurwa zvakaenzanirana kuita furasiki itsva yetsika, uye svikiro itsva yakawedzerwa.Kuwanda kwesero kwakacherechedzwa zuva raitevera kuti zvionekwe kana mvura yaidiwa.

Kana iyo sero mamiriro asina kunaka, nzira ye centrifugal ndima inofanira kushandiswa.Chekutanga, kumiswa kwesero kunotamirwa kune iyocentrifuge chubhu, centrifuged pa 1000rpm ye 5 min, ipapo supernatant inoraswa, sero rinoputika rinoparadzirwa zvinyoronyoro, uye masero anomiswa zvakare nemhepo itsva.Pakupedzisira, huwandu hwakakodzera hwekumiswa kwesero hunotorwa neshanga, huchiiswa mune hutsvatsika bhodhoro, uye chiyero chakakodzera chemukati mutsva chinowedzerwa.Ramba uchirima.

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